Atlas of Human Semen Examination by Donatella Paoli & Francesco Lombardo & Andrea Lenzi

Atlas of Human Semen Examination by Donatella Paoli & Francesco Lombardo & Andrea Lenzi

Author:Donatella Paoli & Francesco Lombardo & Andrea Lenzi
Language: eng
Format: epub
ISBN: 9783030399986
Publisher: Springer International Publishing


staining solution 1 (eosinophilic xanthene);

staining solution 2 (basophilic thiazine).

It produces stains similar to those achieved using May-Grünwald-Giemsa staining.

4.2.3 Papanicolau Staining for Sperm Cells

This technique uses a nuclear dye (Harris’ haematoxylin) and two cytoplasmic dyes [Orange G6 and EA-50 (eosin-alcohol)], all readily available on the market. The dyes work together to stain the nuclei dark grey-blue and the cytoplasm pink or orange.

5 Chromatin Aspect

5.1 Chromatin Organization in Human Sperm



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